Antioxidant and antiulcer potential of aqueous leaf extract of Kigelia africana against ethanol-induced ulcer in rats

Authors

  • Matheus M dos Santos Departamento de Química, Centro de Ciências Naturais e Exatas, Programa de Pós-graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil
  • Mary T Olaleye Department of Biochemistry, Federal University of Technology, Akure, Nigeria
  • Rafael P Ineu Departamento de Química, Centro de Ciências Naturais e Exatas, Programa de Pós-graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil
  • Aline A Boligon Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil
  • Margareth L Athayde Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil
  • Nilda BV Barbosa Departamento de Química, Centro de Ciências Naturais e Exatas, Programa de Pós-graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil
  • João Batista Teixeira Rocha Departamento de Química, Centro de Ciências Naturais e Exatas, Programa de Pós-graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil

Keywords:

Kigelia africana, gastric damage, antioxidant activity, flavonoids, ethanol, antiulcerogenic potential

Abstract

Ethnobotanical claims regarding Kigelia africana reported antiulcer properties as part of its medicinal application. In this work, aqueous leaf extract from K. africana was investigated for its phytochemical constituents and antiulcer potential against ethanol-induced ulcer in rats. The participation of oxidative stress on ethanol-induced ulcer and the potential protective antioxidant activity of K. africana extracts were investigated by determining vitamin C and thiobarbituric acid reactive species (TBARS) contents in the gastric mucosa of rats. The HPLC analysis showed the presence of gallic acid, chlorogenic acid, caffeic acid and also the flavonoids rutin, quercetin and kaempferol in the aqueous plant extract. Oral treatment with K. africana extract (1.75; 3.5; 7 and 14 mg/kg) one hour after ulcer induction with ethanol decreased in a dose dependent manner the ulcer index. Ethanol increased significantly stomachal TBARS levels and decreased vitamin C content when compared to the control animals. K. africana blunted the ethanol-induced oxidative stress and restored vitamin C content to the control levels. The present results indicate that the aqueous leaf extract from K. africana possesses antiulcer potential. The presence of flavonoids in plant extract suggests that its antiulcerogenic potential is associated with antioxidant activity. Of particular therapeutic potential, K. africana was effective against ethanol even after the induction of ulcer, indicating that it can have protective and curative effects against gastric lesion.

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Published

2014-03-31

How to Cite

dos Santos, M. M., Olaleye, M. T., Ineu, R. P., Boligon, A. A., Athayde, M. L., Barbosa, N. B., & Rocha, J. B. T. (2014). Antioxidant and antiulcer potential of aqueous leaf extract of Kigelia africana against ethanol-induced ulcer in rats. EXCLI Journal, 13, 323–330. Retrieved from https://www.excli.de/index.php/excli/article/view/702

Issue

Vol. 13 (2014)

Section

Original articles

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