Flavonoids and its derivatives from Callistephus chinensis flowers and their inhibitory activities against alpha-glucosidase

Authors

  • Xiaoshu Zhang School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Zhenting Liu Chifeng MinYi Natural Anthocyanins Company Limited. Inner Mongolia Autonomous Region, 024000, China
  • Xiuli Bi School of Life Science, Liaoning University, Shenyang 110036, China
  • Jingxin Liu Chifeng MinYi Natural Anthocyanins Company Limited. Inner Mongolia Autonomous Region, 024000, China
  • Wei Li School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Yuqing Zhao School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China

Keywords:

Callistephus chinensis flowers, stepwise polarity fractions, HPLC, alpha-glucosidase

Abstract

Inhibitors of carbohydrate-hydrolysing enzymes play an important role for the treatment of diabetes. One of the therapeutic methods for decreasing of postprandial hyperglycemia is to retard absorption of glucose by the inhibition of carbohydrate- hydrolysing enzymes, such as α-glucosidase, in the digestive organs. To investigate the therapeutic potential of compounds from natural sources, Callistephus chinensis flowers (CCF) were tested for inhibition of α-glucosidase, and acarboes was used as the positive control. The 70 % ethanol extract of CCF exhibited significant α-glucosidase inhibitory activities with IC50 value of 8.14 μg/ml. The stepwise polarity fractions of CCF were tested further for in vitro inhibition of α-glucosidase. The ethyl acetate (EtOAc) fraction exhibited the most significant inhibitory activity. Eight pure compounds, apigenin, apigenin-7-O-β-D- glucoside, kaempferol, hyperin, naringenin, quercetin, luteolin, and kaempferol-7-O-β-D- glucoside, were isolated (using enzyme assay-guide fractionation method) from the EtOAc fraction. Among these, quercetin was the most active one (IC50 values 2.04 μg/ml), and it appears that the inhibiting percentages are close to acarbose (IC50 values 2.24 μg/ml), the positive control, on α-glucosidase inhibition. HPLC/UV analysis indicated that the major components of CCF are kaempferol, hyperin and quercetin. The presented results revealed that CCF containing these eight flavonoids could be a useful natural source in the development of a novel α-glucosidase inhibitory agent against diabetic complications.

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Published

2013-11-19

How to Cite

Zhang, X., Liu, Z., Bi, X., Liu, J., Li, W., & Zhao, Y. (2013). Flavonoids and its derivatives from Callistephus chinensis flowers and their inhibitory activities against alpha-glucosidase. EXCLI Journal, 12, 956–966. Retrieved from https://www.excli.de/index.php/excli/article/view/1206

Issue

Vol. 12 (2013)

Section

Original articles

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